Details

Autor(en) / Beteiligte

• Wang, Junping
• Wen, Ying
• Huo, Fangjun
• Yin, Caixia

Titel

A highly sensitive fluorescent probe for hydrogen sulfide based on dicyanoisophorone and its imaging in living cells

Ist Teil von

• Sensors and actuators. B, Chemical, 2019-09, Vol.294, p.141-147

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2019

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• [Display omitted] •A dicyanoisophorone-based fluorescent probe was developed for sensing H2S with a large Stokes shift (155 nm).•The probe showed faster time response and high sensitivity for H2S.•The probe can be used to detect exdogenous and endogenous H2S in living HeLa cells. Due to the importance of hydrogen sulfide (H2S) in physiology and pathology, the development of fluorescence probes for the detection of H2S has attracted extensive attention of researchers. We developed a H2S fluorescence probe (DCN-S) based on dicyanoisopentanone using dithioether and ester groups as the reaction sites. Under the nucleophilic substitution of H2S, disulfide bond of DCN-S was broken. Then the probe changed into dicyanoisophorone derivative and emitted an orange fluorescence band from 530 nm to 700 nm (centred 580 nm). Upon response for H2S, DCN-S exhibited high sensitivity (88 nM detection limit) and large stokes shift (155 nm). Importantly, the probe also was successfully used for endogenous and exogenous imaging of living cells.