Details

Autor(en) / Beteiligte

• Chen, Shengbao

Titel

On the introduction of 15N2 tracer in marine N2 fixation rate assay

Ist Teil von

• Acta oceanologica Sinica, 2018-01, Vol.40 (10), p.42-50

Ort / Verlag

Beijing: Springer Nature B.V

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• N2 fixation plays an essential role in marine biogeochemical cycles by supplying new N.Stable isotope tracer(15N2)assay has been considered as the best approach for measuring in situ N2 fixation rate.Introducing 15N2 in an efficient and clean way,and precisely determining the isotopic enrichment level in the incubation system,is thus fundamental for obtaining true rates.In this study,we show that modified bubble treatment is a recommended practice in introducing 15N2.It can create 15N atomic abundance of substrate N2 in the incubation bottle higher than 10%.Such enrichment is twice higher when compared with gas pre-dissolution treatment.Moreover,modified bubble treatment is featured by much lowered risk of trace metal contamination and less time/labor consuming.We propose that the modified bubble method coupled to stable isotope mass spectrometry should be widely used in measuring N2 fixation rate in aquatic environments.