Acta biomaterialia, 2019-05, Vol.90, p.393-402
2019
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- Autor(en) / Beteiligte
- Titel
- Mesoporous bioactive glass/ɛ-polycaprolactone scaffolds promote bone regeneration in osteoporotic sheep
- Ist Teil von
- Acta biomaterialia, 2019-05, Vol.90, p.393-402
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- [Display omitted] Macroporous scaffolds made of a SiO2-CaO-P2O5 mesoporous bioactive glass (MBG) and ɛ-polycaprolactone (PCL) have been prepared by robocasting. These scaffolds showed an excellent in vitro biocompatibility in contact with osteoblast like cells (Saos 2) and osteoclasts derived from RAW 264.7 macrophages. In vivo studies were carried out by implantation into cavitary defects drilled in osteoporotic sheep. The scaffolds evidenced excellent bone regeneration properties, promoting new bone formation at both the peripheral and the inner parts of the scaffolds, thick trabeculae, high vascularization and high presence of osteoblasts and osteoclasts. In order to evaluate the effects of the local release of an antiosteoporotic drug, 1% (%wt) of zoledronic acid was incorporated to the scaffolds. The scaffolds loaded with zoledronic acid induced apoptosis in Saos 2 cells, impeded osteoclast differentiation in a time dependent manner and inhibited bone healing, promoting an intense inflammatory response in osteoporotic sheep. In addition to an increase in bone fragility and susceptibility to fracture, osteoporosis also hinders the clinical success of endosseous implants and grafting materials for the treatment of bone defects. For the first time, macroporous scaffolds made of mesoporous bioactive glass and ε-caprolactone have been evaluated in a sheep model that mimics the osteoporosis conditions in humans. These implants fostered bone regeneration, promoting new bone formation at both the peripheral and the inner parts of the scaffolds, showing thick trabeculae and a high vascularization degree. Our results indicate that macroporous structures containing highly bioactive mesoporous glasses could be excellent candidates for the regenerative treatment of bone defects in osteoporotic patients.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1742-7061eISSN: 1878-7568DOI: 10.1016/j.actbio.2019.04.019Titel-ID: cdi_proquest_journals_2240141165
- Format
- –
- Schlagworte
- Animals, Apoptosis, Biocompatibility, Bioglass, Biological activity, Biomedical materials, Bone growth, Bone healing, Bone regeneration, Bone Regeneration - drug effects, Disease Models, Animal, Drug Implants - chemistry, Drug Implants - pharmacokinetics, Drug Implants - pharmacology, Female, Glass - chemistry, Humans, Implantation, In vivo methods and tests, Inflammation, Inflammatory response, Macrophages, Mesoporous bioactive glass, Mice, Osteoblasts, Osteoblasts - metabolism, Osteoblasts - pathology, Osteoclastogenesis, Osteoclasts, Osteoclasts - metabolism, Osteoclasts - pathology, Osteogenesis, Osteogenesis - drug effects, Osteoporosis, Osteoporosis - drug therapy, Osteoporosis - metabolism, Osteoporosis - pathology, Phosphorus pentoxide, Polycaprolactone, Polyesters - chemistry, Polyesters - pharmacology, Porosity, RAW 264.7 Cells, Regeneration, Regeneration (physiology), Scaffolds, Sheep, Silicon dioxide, Surgical implants, Time dependence, Vascularization, Zoledronic acid, Zoledronic Acid - chemistry, Zoledronic Acid - pharmacokinetics, Zoledronic Acid - pharmacology