Details

Autor(en) / Beteiligte

• Bobba, Kondapa Naidu
• Binoy, Anupama
• Koo, Seyoung
• Nedungadi, Divya
• Podder, Arup
• Sharma, Amit
• Mishra, Nandita
• Kim, Jong Seung
• Bhuniya, Sankarprasad

Titel

Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Ist Teil von

• Chemical communications (Cambridge, England), 2019-05, Vol.55 (45), p.6429-6432

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H 2 S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H 2 S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells. Endogenous H 2 S-stimulated protonophores released from MitoDP are a vanguard in creating mitochondria-targeted therapies for reducing mitochondrial activity in cancer cells.