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The objective of this study is to analyze whether a combination of biomarkers at different levels of biological complexity could be used to assess the health status of a population of Brachidontes rodriguezii associated to a harbor area in Mar del Plata, Argentina. A battery of biomarkers of general stress was measured in mussels collected from impacted and non-impacted areas. This included: condition index; shell analysis; histopathological alterations; atrophy and integrity of the digestive gland tissue; changes in cell type composition of the digestive gland, and glycogen accumulation. The studied biomarkers were integrated into the Integrative Biological Response (IBR/n) index. Overall, the IBR/n indicated a higher level of stress in mussels located in the harbor area. Thus, this biomarker index is a sensitive analytical tool that could be used to classify the ecotoxicological risk in coastal sites.
•Different biomarkers were used to asses the health status of B. rodriguezii.•Histopathological alterations were found in the digestive gland, gonad and, gills.•Mussels residing in the harbor showed higher MLR/MET, VvBas and CTD values.•Mussels residing in the harbor showed lower values of CI, shell density and Gly.•IBR index indicated higher levels of stress in mussels from the harbor area.