Details

Autor(en) / Beteiligte

• Nowroozi-Nejad, Zohreh
• Bahramian, Bahram
• Hosseinkhani, Saman

Titel

A fast and efficient stabilization of firefly luciferase on MIL-53(Al) via surface adsorption mechanism

Ist Teil von

• Research on chemical intermediates, 2019-04, Vol.45 (4), p.2489-2501

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Stabilization and immobilization on a proper support is essential in processing and production of enzymes. Luciferase is an unstable, thermally sensitive, and light-emitting enzyme that requires stabilization for efficiency and productivity in bio-luminescence reactions. In this work, a known and well-porous framework, MIL-53(Al), was used, for the first time, to support the immobilization of firefly luciferase. Interestingly, all the stability values were improved through the immobilization process, and the content of the loading enzyme on MIL-53(Al) was found to be significant. The results obtained indicated that the stabilization of firefly luciferase on MIL-53(Al) proceeded through the adsorption method. MIL-53(Al) was also characterized by the FT-IR, X-ray diffraction, FE-SEM, TEM, BET, and TGA techniques in order to determine the carboxylic groups and symmetric/asymmetric CO 2 , percentage of crystallinity and its purity, morphology, size, and shape of the MIL-53(Al) particles, and surface-to-area ratio and thermal gravimetry of support. Graphical abstract