Details

Autor(en) / Beteiligte

• Inoue, Y.(National Agricultural Research Center, Tsukuba, Ibaraki (Japan))
• Matsuura, T
• Ohara, T
• Azegami, K

Titel

Sequence analysis of the genome of OP2' a lytic bacteriophage of Xanthomonas oryzae pv. oryzae

Ist Teil von

• Journal of general plant pathology : JGPP, 2006-04, Vol.72 (2), p.104-110

Ort / Verlag

Tokyo: Springer Nature B.V

Erscheinungsjahr

2006

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We sequenced and analyzed the genome of OP2 (MAFF 106404), a bacteriophage that lyses Xanthomonas oryzae pv. oryzae (Xoo). It is 464bp, and the average G + C content is 60.9%. The results suggest that the genome sequence of OP2 is circularly permuted and terminally redundant, like that of coliphages T4 or P22. Sixty-two open reading frames (ORFs) were predicted in the genome. We constructed a circular map of the restriction enzyme sites and a gene map. The genome is clearly divided into four parts according to transcription direction. The directions of 27 ORFs are leftward and those of 35 are rightward. Most leftward ORFs are commonly conserved among many other phages, and some are structure-related or expression-related genes. In contrast, most rightward ORFs contain OP2-specific genes as well as DNA polymerase, helicase, dUTPase, and HNH family endonuclease genes. Nine rightward and 25 leftward ORFs have homology with the ORFs of bacteriophage Bcep1, which lyses Burkholderia cepacia. The leftward-transcribed OP2 genes and their orthologs in Bcep1 conserve a neighboring relationship, although the putative tail fiber gene does not have homology with that of Bcep1. Several ORFs have similarity (We sequenced and analyzed the genome of OP2 (MAFF 106404), a bacteriophage that lyses Xanthomonas oryzae pv. oryzae (Xoo). It is 464bp, and the average G + C content is 60.9%. The results suggest that the genome sequence of OP2 is circularly permuted and terminally redundant, like that of coliphages T4 or P22. Sixty-two open reading frames (ORFs) were predicted in the genome. We constructed a circular map of the restriction enzyme sites and a gene map. The genome is clearly divided into four parts according to transcription direction. The directions of 27 ORFs are leftward and those of 35 are rightward. Most leftward ORFs are commonly conserved among many other phages, and some are structure-related or expression-related genes. In contrast, most rightward ORFs contain OP2-specific genes as well as DNA polymerase, helicase, dUTPase, and HNH family endonuclease genes. Nine rightward and 25 leftward ORFs have homology with the ORFs of bacteriophage Bcep1, which lyses Burkholderia cepacia. The leftward-transcribed OP2 genes and their orthologs in Bcep1 conserve a neighboring relationship, although the putative tail fiber gene does not have homology with that of Bcep1. Several ORFs have similarity (<e-5) to phage-related genes of Xylella fastidiosa and Xanthomonas spp. Exceptionally, the dUTPase gene had 94% identity with Xoo, and no insertions or deletions were detected in it. However, its neighboring regions did not have homology with the Xoo sequence. Although OP2 is a virulent phage of Xoo, some of the genetic characters have features of temperate phages. [PUBLICATION ABSTRACT]