Plant physiology (Bethesda), 2000-09, Vol.124 (1), p.125-133
2000
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- Autor(en) / Beteiligte
- Titel
- Expression of Arabidopsis CAX2 in Tobacco. Altered Metal Accumulation and Increased Manganese Tolerance
- Ist Teil von
- Plant physiology (Bethesda), 2000-09, Vol.124 (1), p.125-133
- Ort / Verlag
- Rockville, MD: American Society of Plant Physiologists
- Erscheinungsjahr
- 2000
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Metal transport from the cytosol to the vacuole is thought to be an important component of ion tolerance and of a plant's potential for use in phytoremediation. The Arabidopsis antiporter CAX2 (calcium exchanger 2) may be a key mediator of this process. CAX2 expression in yeast suppressed both Ca2+ and Mn2+ growth defects. A peptide-specific antibody to the antiporter reacted with a 39-kD protein from plant vacuolar membranes. Tobacco (Nicotiana tabacum) plants expressing CAX2 accumulated more Ca2+, Cd2+, and Mn2+ and were more tolerant to elevated Mn2+ levels. Expression of CAX2 in tobacco increased Cd2+ and Mn2+ transport in isolated root tonoplast vesicles. These results suggest that CAX2 has a broad substrate range and modulation of this transporter may be an important component of future strategies to improve plant ion tolerance.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.124.1.125Titel-ID: cdi_proquest_journals_218633041
- Format
- –
- Schlagworte
- Adaptation to environment and cultivation conditions, Agronomy. Soil science and plant productions, Antibodies, Antiporters, Antiporters - genetics, Antiporters - metabolism, Arabidopsis - genetics, Arabidopsis - metabolism, Biological and medical sciences, Biological Transport, Active, Blotting, Northern, Cadmium - metabolism, Calcium - metabolism, Calcium-Binding Proteins - genetics, Calcium-Binding Proteins - metabolism, Cation Transport Proteins, Cations, Divalent, Cell membranes, Cell physiology, Cells, Cultured, Cytosol - metabolism, Electrophoresis, Polyacrylamide Gel, Environmental Stress and Adaptation, Fundamental and applied biological sciences. Psychology, Genetics and breeding of economic plants, Manganese - metabolism, Nicotiana - genetics, Nicotiana - metabolism, Phenotype, Plant physiology and development, Plant roots, Plants, Plants, Genetically Modified, Plants, Toxic, Plasma membrane and permeation, Radiocarbon, RNA, Tonoplast, Transgenic plants, Vacuoles - metabolism, Varietal selection. Specialized plant breeding, plant breeding aims, Yeasts, Yeasts - genetics, Yeasts - metabolism