Details

Autor(en) / Beteiligte

• Le, Thien-Kim
• Park, Jong Hyun
• Choi, Da Som
• Lee, Ga-Young
• Choi, Woo Sung
• Jeong, Ki Jun
• Park, Chan Beum
• Yun, Chul-Ho

Titel

Solar-driven biocatalytic C-hydroxylation through direct transfer of photoinduced electrons

Ist Teil von

• Green chemistry : an international journal and green chemistry resource : GC, 2019-02, Vol.21 (3), p.515-525

Ort / Verlag

Cambridge: Royal Society of Chemistry

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Despite the immense potential of P450s, the dependence on the nicotinamide cofactor (NADPH) and NADPH-P450 reductase (CPR) limits their employment in the chemical industry. Here, we present a visible light-driven platform for biocatalytic C-hydroxylation reactions using natural flavin molecules, especially flavin mononucleotide, as a photosensitizer. By employing visible light as a source of energy instead of the nicotinamide cofactor, the bacterial CYP102A1 heme domain was successfully applied for photobiocatalytic C-hydroxylation of 4-nitrophenol and lauric acid - in the absence of NADPH and CPR. We present a proof of concept that the photoactivation of flavins is productively coupled with the direct transfer of photoinduced electrons to the P450 heme iron, achieving photobiocatalytic C-hydroxylation reactions. Photoactivation of flavins is coupled productively with the direct transfer of photoinduced electrons to P450s to achieve photobiocatalytic C-hydroxylation reactions in the absence of nicotinamide cofactors.