Details

Autor(en) / Beteiligte

• KISHI, Motoko
• TANABE, James
• SCHMELZER, James D
• LOW, Phillip A

Titel

Morphometry of Dorsal Root Ganglion in Chronic Experimental Diabetic Neuropathy

Ist Teil von

• Diabetes (New York, N.Y.), 2002-03, Vol.51 (3), p.819-824

Ort / Verlag

Alexandria, VA: American Diabetes Association

Erscheinungsjahr

2002

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Morphometry of Dorsal Root Ganglion in Chronic Experimental Diabetic Neuropathy Motoko Kishi , James Tanabe , James D. Schmelzer and Phillip A. Low From the Department of Neurology, Mayo Clinic, Rochester, Minnesota Abstract Chronic hyperglycemia results in a predominantly sensory neuropathy. Recent studies suggest that dorsal root ganglion (DRG) neurons comprise a specific target and may be responsible for the important complication of diabetic sensory neuropathy, since hyperglycemia for longer than 6 months results in a vacuolar ganglionopathy with associated radiculopathy and distal sensory neuropathy. We undertook morphometric analysis of L5 DRG neurons in seven diabetic rats and six age- and sex-matched littermates. Nerve conduction studies were also performed, and neuropathy was confirmed. Diabetes was induced with streptozotocin; duration of diabetes was 12 months. The DRG count for control rats was 15,304 ± 991 neurons. Two of seven diabetic DRG counts were reduced, but the group mean count at 14,847 ± 1,524 was not significantly reduced. The number of small neurons (type B) considerably exceeded that of large neurons (type A), at a ratio of 71:29. The percentage of large cells was significantly reduced in diabetic compared with control rats ( P = 0.01). The large-diameter population can be subdivided into two groups; with this subdivision, the number of neurons <50 μm was not reduced in samples from diabetic rats, but the neurons of largest size (≥50 μm) were significantly reduced (by 41%). Footnotes Address correspondence and reprint requests to Phillip A. Low, Department of Neurology, Mayo Clinic, 200 First St. SW, Rochester, MN 55905. E-mail: low{at}mayo.edu . Received for publication 21 February 2001 and accepted in revised form 30 November 2001. DRG, dorsal root ganglion; STZ, streptozotocin. DIABETES