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Biochemical, molecular, and elemental profiling of Withania somnifera L. with response to zinc stress
Ist Teil von
Environmental science and pollution research international, 2019-02, Vol.26 (4), p.4116-4129
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2019
Quelle
SpringerLink
Beschreibungen/Notizen
Zn stress seriously induces various toxic responses in
Withania somnifera
L., when accumulated above the threshold level which was confirmed by investigating the responses of protein, expression of antioxidant enzymes, and elemental profiling on accumulation of Zn. Zn was supplemented in the form of ZnSO
4
(0, 25, 50, 100, and 200 μM) through MS liquid medium and allowed to grow the
in vitro
germinated plants for 7 and 14 days. The study revealed that when the application of Zn increased, a significant reduction of growth characteristics was noticed with alterations of proteins (both disappearance and
de novo
synthesis). The activity of CAT, SOD, and GPX were increased up to certain concentrations and then declined, which confirmed through in-gel activity under different treatments. RT-PCR was conducted by taking three sets of genes from CAT (
RsCat
,
Catalase1
,
Cat1
) and SOD (
SodCp
,
TaSOD1.2
,
MnSOD
) and found that gene
RsCat
from CAT and
MnSOD
from SOD have shown maximum expression of desired genes under Zn stress, which indicate plant’s stress tolerance mechanisms. The proton-induced X-ray emission study confirmed an increasing order of uptake of Zn in plants by suppressing and expressing other elemental constituents which cause metal homeostasis. This study provides insights into molecular mechanisms associated with Zn causing toxicity to plants; however, cellular and subcellular studies are essential to explore molecule-molecule interaction during Zn stress in plants.