Anticancer research, 2018-06, Vol.38 (6), p.3273-3282
2018
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- Autor(en) / Beteiligte
- Titel
- An Agonistic Antibody to EPHA2 Exhibits Antitumor Effects on Human Melanoma Cells
- Ist Teil von
- Anticancer research, 2018-06, Vol.38 (6), p.3273-3282
- Ort / Verlag
- Greece: International Institute of Anticancer Research
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- EPH receptor A2 (EPHA2) is highly expressed in aggressive types of human cancer, and is expected to be an excellent target molecule for antibody treatments. In this study, we investigated the therapeutic potential of antibody to EPHA2 against melanoma in vitro. We generated three monoclonal antibodies (mAbs) to EPHA2 and examined cell-surface expression by flow cytometry. To investigate the ability to inhibit tumor cell migration therapy with mAbs to EPHA2, we performed a wound scratch assay and invasion assay. We investigated the therapeutic effects of immunotoxins consisting of toxin-conjugated EPHA2 mAbs. All human melanoma cell lines studied expressed EPHA2. Like natural ligand ephrin-A1, one of EPHA2 mAbs, SHM16, inhibited metastatic behavior of cells, such as migration and invasion. In addition, drastic growth inhibition and cytotoxicity were found using immunotoxin-conjugated SHM16. These observations indicate a promising role for EPHA2 as a target in antibody treatments for melanoma, and demonstrate the potential therapeutic effects of an agonistic antibody to EPHA2.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0250-7005eISSN: 1791-7530DOI: 10.21873/anticanres.12592Titel-ID: cdi_proquest_journals_2137115252
- Format
- –
- Schlagworte
- Animals, Antibodies, Monoclonal - immunology, Antibodies, Monoclonal - pharmacology, Anticancer properties, Antitumor activity, Cancer, Cell adhesion & migration, Cell Line, Tumor, Cell migration, Cell Movement - drug effects, Cell Proliferation - drug effects, Cell surface, Cell Survival - drug effects, Cytotoxicity, EphA2 protein, Flow cytometry, Growth inhibition, Humans, Immunotoxins, In vitro methods and tests, Melanoma, Melanoma - genetics, Melanoma - metabolism, Melanoma - pathology, Metastases, Mice, Inbred BALB C, Monoclonal antibodies, Receptor, EphA2 - agonists, Receptor, EphA2 - immunology, Receptor, EphA2 - metabolism, RNA Interference, Skin Neoplasms - genetics, Skin Neoplasms - metabolism, Skin Neoplasms - pathology, Toxicity