Details

Autor(en) / Beteiligte

• Valdovinos, Hector F.
• Hernandez, Reinier
• Goel, Shreya
• Graves, Stephen
• Barnhart, Todd
• Cai, Weibo
• Nickles, Robert J.
• Mendez, Ivan Miguel Rosado
• Fossion, Ruben
• Massillon-JL, Guerda
• Avila-Rodriguez, Miguel A.
• López-Pérez, Danna Oassis

Titel

Auger electron-based targeted radioimmunotherapy with 58mCo, a feasibility study

Ist Teil von

• AIP Conference Proceedings, 2016, Vol.1747 (1)

Ort / Verlag

Melville: American Institute of Physics

Erscheinungsjahr

2016

Beschreibungen/Notizen

• Co-58m (t 1/2 =9.10 h, 100% IC) is an attractive radionuclide for localized delivery of radiation dose due to its emission of Auger and conversion electrons with high LET in water (2-18 keV/µm). 58mCo decays to a positron-emitting daughter, 58gCo (t 1/2 =70.86 d, 14.9% β+), which can be readily detected by Positron Emission Tomography (PET) and hence can be employed to verify the biodistribution of the parent isotope post-treatment in vivo. In this work, we describe 1) the targetry and radiochemistry involved in the production of high specific activity 58mCo; and 2) the results of a pilot radiotherapy study using a 58mCo-labeled anti-CD105 antibody (TRC105) in a mouse model of mammary carcinoma. The presented methods allow for the production of 14.3±3.2 MBq/µAh of 58mCo with a separation efficiency of 96±2% and a specific activity with NOTA of 25±6 GBq/µmol. We have produced ∼640 MBq of 58mCo-NOTA-TRC105, with a specific activity of 1.2 MBq/µg, in 800 µL of PBS ready for targeted radioimmunotherapy, which we employed in a group of 4 mice. Post-therapy biodistribution was quantified in vivo via PET imaging of the daughter 58gCo.