Details

Autor(en) / Beteiligte

• Lv, Yun
• Dan Cheng
• Dongdong Su
• Chen, Mei
• Yin, Bin-Cheng
• Yuan, Lin
• Zhang, Xiao-Bing

Titel

Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes

Ist Teil von

• Chemical science (Cambridge), 2018-10, Vol.9 (39), p.7606-7613

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2018

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O ˙ ), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI mapping the fluctuation of O ˙ with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes ( and ) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O ˙ in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O ˙ level during the drug-induced nephrotoxicity. Moreover, probe was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O ˙ in complex nephrotoxicity disease systems.