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Alveolar epithelial ion and fluid transport: Polarity of alveolar epithelial cell acid-base permeability
Ist Teil von
American journal of physiology. Lung cellular and molecular physiology, 2002-04, Vol.26 (4), p.L675
Ort / Verlag
Bethesda: American Physiological Society
Erscheinungsjahr
2002
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ）
Beschreibungen/Notizen
We investigated acid-base permeability properties of electrically resistive monolayers of alveolar epithelial cells (AEC) grown in primary culture, AEC monolayers were grown on tissue culture-treated polycarbonate filters. Filters were mounted in a partitioned cuvette containing two fluid compartments (apical and basolateral) separated by the adherent mono-layere, cells were loaded with the pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, and intracellular pH was determined.