Details

Autor(en) / Beteiligte

• Philippe Dje N'Guessan
• Hippenstiel, Stefan
• Etouem, Mirabelle O
• Zahlten, Janine

Titel

Streptococcus pneumoniae induced p38 MAPK- and NF-kappaB-dependent COX-2 expression in human lung epithelium

Ist Teil von

• American journal of physiology. Lung cellular and molecular physiology, 2006-06, Vol.34 (6), p.L1131

Ort / Verlag

Bethesda: American Physiological Society

Erscheinungsjahr

2006

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Streptococcus pneumoniae is a major cause of community-acquired pneumonia and death from infectious diseases in industrialized countries. Lung airway and alveolar epithelial cells comprise an important barrier against airborne pathogens. Cyclooxygenase (COX)-derived prostaglandins, such as PGE2, are considered to be important regulators of lung function. Herein, we tested the hypothesis that pneumococci induced COX-2-dependent PGE2 production in pulmonary epithelial cells. Pneumococci-infected human pulmonary epithelial BEAS-2B cells released PGE2. Expression of COX-2 but not COX-1 was dose and time dependently increased in S. pneumoniae-infected BEAS-2B cells as well as in lungs of mice with pneumococcal pneumonia. S. pneumoniae induced degradation of IB and DNA binding of NF-[kappa]B. A specific peptide inhibitor of the IB kinase complex blocked pneumococci-induced PGE2 release and COX-2 expression. In addition, we noted activation of p38 MAPK and JNK in pneumococci-infected BEAS-2B cells. PGE2 release and COX-2 expression were reduced by p38 MAPK inhibitor SB-202190 but not by JNK inhibitor SP-600125. We analyzed interaction of kinase pathways and NF-[kappa]B activation: dominant-negative mutants of p38 MAPK isoforms alpha, beta2, gamma, and delta blocked S. pneumoniae-induced NF-[kappa]B activation. In addition, recruitment of NF-[kappa]B subunit p65/RelA and RNA polymerase II to the cox2 promoter depended on p38 MAPK but not on JNK activity. In summary, p38 MAPK- and NF-[kappa]B-controlled COX-2 expression and subsequent PGE2 release by lung epithelial cells may contribute significantly to the host response in pneumococcal pneumonia. [PUBLICATION ABSTRACT]