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The paper aims to explain the effect of laser irradiation on the nonlinear optical (NLO) response of MoS
2
nanosheets. MoS
2
-layered nanostructures were prepared by liquid-phase exfoliation (LPE) in deionized (DI) water. The characterization of MoS
2
dispersion proves the formation of 2-3-layered 2H-MoS
2
nanosheets with less than 100 nm lateral dimension. The NLO response of prepared dispersion was explored using the Z-scan technique with 532 nm continuous wave (CW) laser beam at 30, 50, 70 mW powers. The main finding of this research is that the nonlinear refractive index (
n
2
) of MoS
2
dispersion is a laser-assisted tunable quantity. It has been shown that the nonlinear refractive index of MoS
2
nanosheets is altered up to 48% of its initial value only by 1-h laser irradiation with 30 mW power.