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Based on a multi-domain system involving an up-gradient permeable reactive barrier (PRB) and a down-gradient aquifer, analytical solutions were developed to solve the multispecies solute transport. Multi-domains configuration of the transformation approach used in the previous studies is validated by comparing the calculation results from these solutions and numerical results computed by using the numerical reactive transport code. The solutions are calculated and compared with the numerical model results. When the compliance plane is far from the PRB exit face, the aquifer reaction capability was found important for the required thickness calculation of the PRB. A new PRB design equation for multi-species solute transport is developed. For the remediation multiple species sharing a single parental species, the proposed analytical solution provides meaningful designing information for the PRB remediation settlement.