Details

Autor(en) / Beteiligte

• Han, Bing
• Zhang, Yong-Lai
• Zhu, Lin
• Chen, Xu-Hui
• Ma, Zhuo-Chen
• Zhang, Xu-Lin
• Wang, Jian-Nan
• Wang, Wei
• Liu, Yu-Qing
• Chen, Qi-Dai
• Sun, Hong-Bo

Titel

Direct laser scribing of AgNPs@RGO biochip as a reusable SERS sensor for DNA detection

Ist Teil von

• Sensors and actuators. B, Chemical, 2018-10, Vol.270, p.500-507

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •We demonstrate a laser scribing method to fabricate silver nanoparticles (AgNPs) and graphene oxide (GO) based biochips as a reusable SERS sensor.•Programmable laser scribing of the AgNPs@GO composite film enables direct patterning of sensitive SERS channels.•The noncovalent interactions between DNA and graphene mediated controllable trapping and releasing of DNA sequences.•The proposed fabrication method of the SERS-active biochips reveals great potential for biomolecular sensing and genetic engineering. The combination of surface-enhanced Raman spectroscopy (SERS) technology with microfluidics makes it possible to diagnose genetic disease through label-free on-chip DNA detection. However, open problems including the integration of SERS substrate with microfluidic devices, controllable trapping and releasing of target molecules are still challenging. Here we demonstrate a facile laser scribing method to fabricate silver nanoparticles (AgNPs) and graphene oxide (GO) based biochips as a reusable SERS sensor for DNA detection. Programmable laser scribing of the AgNPs@GO composite film enables direct patterning of sensitive SERS channels that consist of graphene supported AgNPs by exfoliating the composites into hierarchical porous structures. Integrating the SERS-active patterns with a microfluidic chip forms a biochip for allowing SERS detection of DNA. The noncovalent interactions between DNA and graphene mediated controllable trapping and releasing of DNA sequences, enabling efficient on-chip SERS detection and the regeneration of the biochip. The simple, green and cost-effective fabrication of the SERS-active biochips reveals great potential for biomolecular sensing and genetic engineering applications.