Details

Autor(en) / Beteiligte

• Pivovarov, V. D.
• Ryazantsev, D. Yu
• Simonova, M. A.
• Yegorova, T. V.
• Khlgatian, S. V.
• Zavriev, S. K.
• Svirshchevskaya, E. V.

Titel

Immuno-PCR Assay for Quantitation of Antibodies to Epstein–Barr Virus

Ist Teil von

• Molecular biology (New York), 2018-07, Vol.52 (4), p.629-635

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2018

Quelle

SpringerLink

Beschreibungen/Notizen

• Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein–Barr virus (EBV) in the human blood serum. EBV nuclear protein 1 fragment (pEBV) was expressed in Escherichia coli . A synthetic single-stranded deoxyribonucleotide was conjugated to streptavidin, and the conjugate was used to detect рEBV–IgG1–biotin complexes by qiPCR. The IgG1 titers determined by qiPCR were compared to the results of enzyme-linked immunosorbent assay (ELISA). The sensitivity of qiPCR was one order of magnitude higher than that of ELISA. Thus, a highly sensitive qiPCR-based assay was developed to quantitate antibodies specific to the recombinant EBV antigen.