Details

Autor(en) / Beteiligte

• Rengaraj, Saravanan
• Cruz-Izquierdo, Álvaro
• Scott, Janet L.
• Di Lorenzo, Mirella

Titel

Impedimetric paper-based biosensor for the detection of bacterial contamination in water

Ist Teil von

• Sensors and actuators. B, Chemical, 2018-07, Vol.265, p.50-58

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Simple and low-cost, paper-based probe for detection of bacteria in water.•Electrode surface functionalised with carboxyl groups and with the lectin concanavalin A.•Sensor dynamic range: 103–106 CFU mL−1. According to the World Health Organisation, worldwide waterborne diseases are responsible for nearly two million human deaths annually. Rapid and at-site screen of pathogenic microorganisms in drinking water can help to markedly reduce this number. Here we report an innovative, simple and low-cost, paper-based probe for detection of bacteria in water, fabricated by screen printing carbon electrodes onto hydrophobic paper. Electrochemical characterization of the printed electrodes confirmed fast-electron transfer, with an estimated electroactive surface area of 0.25 cm2. The electrode surface was functionalised with carboxyl groups, prior to covalent immobilization of the lectin Concanavalin A (Con A), used as the biorecognition element. The system was then tested as an impedimetric sensor for bacteria in water. A linear increase in the probe charge transfer resistance was observed for bacterial concentrations ranging from 103 to 106 CFU mL−1, with an estimated lower detection limit of 1.9 × 103 CFU mL−1. Considering its remarkable simplicity, cost-effectiveness and biodegradability, the sensor here reported could be an attractive solution for portable testing kits that address the challenges of traditional time-consuming and expensive lab-based analyses.