Toxicology in vitro, 2018-03, Vol.47, p.129-136
2018
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- Autor(en) / Beteiligte
- Titel
- In vitro antitumor effect of a lignan isolated from Combretum fruticosum, trachelogenin, in HCT-116 human colon cancer cells
- Ist Teil von
- Toxicology in vitro, 2018-03, Vol.47, p.129-136
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2018
- Quelle
- ScienceDirect
- Beschreibungen/Notizen
- The use of natural products in therapeutics has been growing over the years. Lignans are compounds with large pharmaceutical use, which has aroused interest in the search for new drugs to treat diseases. The present study evaluated the cytotoxicity of (−)-trachelogenin, a dibenzylbutyrolactone type lignan isolated from Combretum fruticosum, against several tumor and non-tumor cell lines using the MTT assay and its possible mechanism of action. (−)-Trachelogenin showed IC50 values ranging of 0.8–32.4μM in SF-295 and HL-60 cell lines, respectively and IC50 values >64μM in non-tumor cell lines. (−)-trachelogenin persistently induced autophagic cell death, with cytoplasmic vacuolization and formation of autophagosomes mediated by increasing LC3 activation and altering the expression levels of Beclin-1. •(−)-Trachelogenin was isolated from Combretum fruticosum.•Cytotoxicity of (−)-Trachelogenin was evaluated against tumor and non-tumor cells.•(−)-Trachelogenin induced cytoplasmic vacuolization and cell death.•Treated HCT-116 cells had chances in specific markers of autophagy.•The treatment with (−)-Trachelogenin induced autophagy in HCT-116 cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0887-2333eISSN: 1879-3177DOI: 10.1016/j.tiv.2017.11.014Titel-ID: cdi_proquest_journals_2067368336
- Format
- –
- Schlagworte
- 4-Butyrolactone - adverse effects, 4-Butyrolactone - analogs & derivatives, 4-Butyrolactone - chemistry, 4-Butyrolactone - isolation & purification, 4-Butyrolactone - pharmacology, Anticancer, Anticancer properties, Antineoplastic Agents, Phytogenic - adverse effects, Antineoplastic Agents, Phytogenic - chemistry, Antineoplastic Agents, Phytogenic - isolation & purification, Antineoplastic Agents, Phytogenic - pharmacology, Antitumor activity, Autophagosomes - drug effects, Autophagosomes - pathology, Autophagy, Autophagy - drug effects, Beclin-1 - agonists, Beclin-1 - metabolism, Biotechnology, Brazil, Cell death, Cell Line, Cell Line, Tumor, Cell Survival - drug effects, Colon, Colon cancer, Colonic Neoplasms - drug therapy, Colonic Neoplasms - pathology, Colorectal cancer, Combretum, Combretum - chemistry, Combretum - growth & development, Combretum fruticosum, Cytotoxicity, Drug Discovery, Ethnopharmacology, HCT116 Cells, Humans, Inhibitory Concentration 50, Lignans, Medicine, Traditional, Microtubule-Associated Proteins - agonists, Microtubule-Associated Proteins - metabolism, Molecular Structure, Natural products, Neoplasm Proteins - agonists, Neoplasm Proteins - metabolism, Phagosomes, Pharmaceuticals, Plant Stems - chemistry, Plant Stems - growth & development, Toxicity, Tumor cell lines, Tumors, Vacuoles - drug effects, Vacuoles - pathology