Details

Autor(en) / Beteiligte

• Figueroa-Miranda, Gabriela
• Feng, Lingyan
• Shiu, Simon Chi-Chin
• Dirkzwager, Roderick Marshall
• Cheung, Yee-Wai
• Tanner, Julian Alexander
• Schöning, Michael Josef
• Offenhäusser, Andreas
• Mayer, Dirk

Titel

Aptamer-based electrochemical biosensor for highly sensitive and selective malaria detection with adjustable dynamic response range and reusability

Ist Teil von

• Sensors and actuators. B, Chemical, 2018-02, Vol.255, p.235-243

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Novel electrochemical aptamer-sensor for the detection of the malaria specific biomarker PfLDH.•Adjustable response range for PfLDH by changing analyte pH in accordance to the isoelectric point of the target protein.•Electrochemical aptasensor with high sensitivity and detection limit among the lowest reported.•Regeneration of electrochemical aptasensor is demonstrated without loss of sensitivity. Malaria infection remains a significant risk for much of the population of tropical and subtropical areas, particularly in developing countries. Therefore, it is of high importance to develop sensitive, accurate and inexpensive malaria diagnosis tests. Here, we present a novel aptamer-based electrochemical biosensor (aptasensor) for malaria detection by impedance spectroscopy, through the specific recognition between a highly discriminatory DNA aptamer and its target Plasmodium falciparum lactate dehydrogenase (PfLDH). Interestingly, due to the isoelectric point (pI) of PfLDH, the aptasensor response showed an adjustable detection range based on the different protein net-charge at variable pH environments. The specific aptamer recognition allows sensitive protein detection with an expanded detection range and a low detection limit, as well as a high specificity for PfLDH compared to analogous proteins. The specific feasibility of the aptasensor is further demonstrated by detection of the target PfLDH in human serum. Furthermore, the aptasensor can be easily regenerated and thus applied for multiple usages. The robustness, sensitivity, and reusability of the presented aptasensor make it a promising candidate for point-of-care diagnostic systems.