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This study investigated the effect of organic and conventional orange juice consumption on biochemical parameters of Wistar rats. Eighteen rats were divided into 3 groups: Control Group (CG), receiving a balanced diet and water; Organic Juice Group (OJG), receiving a balanced diet, water, and organic orange juice (15 mL/day), and; Conventional Juice Group (CJG), receiving a balanced diet, water, and conventional orange juice (15 mL/day). After 30 days, the animals were anesthetized and sacrificed. Blood was collected to obtain plasma for analysis. The Folin-Ciocalteu reagent method was used to determine the content of soluble polyphenols of the juices. Organic orange juice exhibited a significantly higher concentration of soluble polyphenols compared with conventional juice (p<0.0001). After the intervention, there were no statistical differences in glucose levels, total and LDL cholesterol, and triglycerides among the groups. There was a significant higher HDL-cholesterol in OJG compared to GC (p=0.04). It is suggested that the higher HDL levels in the group receiving the organic juice is related to the greater concentration of polyphenol found in our results.