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Characterization of Late Blight Resistance Potato Breeding Lines with the RB Gene from Solanum bulbocastanum
Ist Teil von
American journal of potato research, 2018-10, Vol.95 (5), p.564-574
Ort / Verlag
New York: Springer US
Erscheinungsjahr
2018
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Late blight, caused by
Phytophthora infestans
, is an important disease of potato (
Solanum tuberosum
). Previous work has demonstrated that the wild diploid potato species
Solanum bulbocastanum
showed resistance to all known genotypes of
P. infestans
. In this study, the RB gene (also known as
Rpi-blb1
) from
S. bulbocastanum
was transformed and expressed into conventionally bred, late blight resistant breeding lines to evaluate the effect of pyramiding late blight resistance genes. All RB potato transformation events were confirmed by polymerase chain reaction and RB transcription was detected by reverse transcriptase-polymerase chain reaction. Multiple
P. infestans
genotypes were used to evaluate foliar host plant resistance using a greenhouse whole plant bioassay (WPB) and field trials. The results of the WPB were dependent on different pathogen genotypes. The RB lines derived from MSM171-A late blight resistant (LBR) parental line showed less foliar late blight than other RB lines. Field trials indicated most RB lines from three LBR parental lines had lower RAUDPC values than their parent and susceptible check line. Our results suggest that stacking of R-genes may be a viable strategy to obtain late blight resistant potato varieties.