Details

Autor(en) / Beteiligte

• Crosbie, Jeffrey C
• Anderson, Robin
• Rothkamm, Kai
• Cann, Leonie
• Restall, Tina
• Ruwanpura, Saleela
• Meachem, Sarah
• Yagi, Naoto
• Svalbe, Imants
• Lewis, Robert A
• Williams, Bryan M
• Rogers, Peter A W

Titel

CELLULAR RESPONSES OF NORMAL SKIN TISSUE AND EMT-6 TUMOUR INOCULATED IN MICE FOLLOWING HIGH DOSE SYNCHROTRON MICROBEAM RADIATION THERAPY

Ist Teil von

• Australasian physical & engineering sciences in medicine, 2008-12, Vol.31 (4), p.402

Ort / Verlag

Dordrecht: Springer Nature B.V

Erscheinungsjahr

2008

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Mice were culled when the maximum length of the tumour reached 11 mm. In other MRT experiments, dorsal skinflaps and EMT-6.5 tumours were irradiated and tissues collected for immunohistochemical studies at different time points over the following 5 days. Immunohistochemistry for phosphorylated ãH2AX (a surrogate marker for DNA DSBs) demonstrated nuclear localisation within cells situated in zones through the tissues that correlated well with microbeam spacing. ãH2AX-positive cells were readily detectable from 6hrs to at least 4 days post MRT, suggesting that cells receiving very high levels of radiation fail to repair all DNA double strand breaks.