Environmental toxicology and pharmacology, 2017-12, Vol.56, p.121-128
2017
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- Autor(en) / Beteiligte
- Titel
- In vitro assessment of neurotoxicity and neuroinflammation of homemade MWCNTs
- Ist Teil von
- Environmental toxicology and pharmacology, 2017-12, Vol.56, p.121-128
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- [Display omitted] •PMWCNT and fMWCNT triggered cyto- and geno-toxic effects in neuronal-like cells.•IL-1β, IL-6 and TNFα increases underlined the neuroinflammation MWCNT-induced.•ROS overproduction MWCNT-driven caused neurotoxicity and neuroinflammation.•Despite the different shape, the harmful effects of p-e f MWCNT were overlapped.•Effects of MWCNTs-cells interactions was highlighted by Raman spectrometry. Multi walled carbon nanotubes (MWCNTs) activate pathways involved in cytotoxicity, genotoxicity and inflammation. Inhaled MWCNTs are translocated to extra pulmonary organs and their hydrophobicity allows them to cross the blood–brain barrier (BBB). Further exposure of central nervous system (CNS) occurs via olfactory neurons. Using differentiated SH-SY5Y, we studied the neurotoxicity and neuroinflammation of pristine and functionalised MWCNTs. ROS overproduction was dose- and time-dependent (P<0.01) and was related to mitochondrial impairment, DNA damage and decreased viability (P<0.05). Transcript levels of TNFα, IL-1β and IL-6 increased, as confirmed by an ELISA test. Raman spectra were acquired to assess MWCNT-cells interactions. The almost superimposable pro-oxidant activity of both CNTs could be imputable to excessive lengths with regard to the pristine MWCNTs and to the eroded surface, causing increased reactivity, with regard to functionalised MWCNTs. Considering the ease with which lightweight MWCNTs aerosolize and the increased production, the results underlined the potential onset of neurodegenerative diseases, due to unintentional MWCNT exposure.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1382-6689eISSN: 1872-7077DOI: 10.1016/j.etap.2017.09.005Titel-ID: cdi_proquest_journals_2039275663
- Format
- –
- Schlagworte
- Blood-Brain Barrier, Brain, Carbon, Cell Line, Cell Survival - drug effects, Cells, Central nervous system, Cytotoxicity, Deoxyribonucleic acid, Differentiated SH-SY5Y cells, DNA, DNA Damage, Dose-Response Relationship, Drug, Enzyme-linked immunosorbent assay, Exposure, Gene Expression Regulation - drug effects, Genotoxicity, Humans, Hydrophobicity, Inflammation, Inflammation - genetics, Inflammation - metabolism, Interleukin 6, Interleukin-1beta - genetics, Interleukin-6 - genetics, Mitochondria - drug effects, Mitochondria - genetics, Mitochondrial DNA, Multi wall carbon nanotubes, Multi walled carbon nanotubes (MWCNTs), Nanotechnology, Nanotubes, Nanotubes, Carbon - toxicity, Neurodegenerative diseases, Neuroinflammation, Neurological diseases, Neurotoxicity, Olfactory neurons, Organs, Oxidative damage, Raman spectra, Raman spectroscopy, Reactive oxygen species, Reactive Oxygen Species - metabolism, Time dependence, Time Factors, Toxicity, Transcription, Tumor Necrosis Factor-alpha - genetics, Tumor necrosis factor-α, Viability