Details

Autor(en) / Beteiligte

• Chen-Cheng, Chou
• Mao-Sheng, Huang
• Hsieh, Kue-Hsiung
• Chiang, Bor-Luen

Titel

Reduced IL-12 level correlates with decreased IFN-(gamma) secreting T cells but not natural killer cell activity in asthmatic children

Ist Teil von

• Annals of allergy, asthma, & immunology, 1999-05, Vol.82 (5), p.479

Ort / Verlag

Palatine: American College of Allergy and Immunology

Erscheinungsjahr

1999

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• BACKGROUND: Accessory cells such as macrophages and natural killer cells, and their cytokines such as IL-10, IL-12, and IFN-gamma have been suggested to play a critical role in the development of T helper cells. OBJECTIVE: Both natural killer cells and peripheral blood mononuclear cells were isolated and stimulated for their ability in producing cytokines. In addition, the percentage of IFN-gamma-secreting cells was analyzed with the method of intracellular staining. RESULTS: The data suggested (1) no significant difference between asthmatic children and normal controls in number, cytotoxicity, and IFN-gamma production of purified NK cells; (2) decreased secretion of IL-12 by stimulated peripheral blood mononuclear cells in asthmatic children compared with normals (P < .05); (3) decreased production of IFN-gamma by PBMC from asthmatic children compared with normals (P < .05); and (4) intracellular expressed IFN-gamma level was lower in CD4+ T cells of asthmatic children (P < .05). CONCLUSION: The results suggested that IL-12 produced predominantly by macrophages and associated decreased IFN-gamma-secreting CD4+ T cells play a critical role in the pathogenesis of asthma.