Proceedings of the National Academy of Sciences - PNAS, 2010-01, Vol.107 (2), p.616-621
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Engineering the Cell Wall by Reducing De-Methyl-Esterified Homogalacturonan Improves Saccharification of Plant Tissues for Bioconversion
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2010-01, Vol.107 (2), p.616-621
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2010
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Plant cell walls represent an abundant renewable source of biofuel and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification), to be subsequently converted by microorganisms into ethanol or other products, is their recalcitrance to enzymatic saccharification. We investigated whether the structure of pectin that embeds the cellulose-hemicellulose network affects the exposure of cellulose to enzymes and consequently the process of saccharification. Reduction of de-methyl-esterified homogalacturonan (HGA) in Arabidopsis plants through the expression of a fungal polygalacturonase (PG) or an inhibitor of pectin methylesterase (PMEI) increased the efficiency of enzymatic saccharification. The improved enzymatic saccharification efficiency observed in transformed plants could also reduce the need for acid pretreatment. Similar results were obtained in PG-expressing tobacco plants and in PMEl-expressing wheat plants, indicating that reduction of de-methyl-esterified HGA may be used in crop species to facilitate the process of biomass saccharification.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.0907549107Titel-ID: cdi_proquest_journals_201437051
- Format
- –
- Schlagworte
- Arabidopsis - enzymology, Arabidopsis - genetics, Arabidopsis - physiology, Aspergillus niger - genetics, Biofuels, Biological Sciences, Biomass, Carboxylic Ester Hydrolases - genetics, Cell Wall - drug effects, Cell Wall - physiology, Cell walls, Cells, Cellulose, Cellulose - metabolism, Cellulose - pharmacology, DNA Primers, Enzymes, Flowers & plants, Gene expression, Genetic Vectors, Hypocotyl - metabolism, Nicotiana - genetics, Nicotiana - physiology, Pectins - chemistry, Pectins - metabolism, Pectins - pharmacology, Plant Cells, Plant Leaves - metabolism, Plant Physiological Phenomena, Plant Proteins - genetics, Plants, Polygalacturonase - metabolism, Polysaccharides - metabolism, Polysaccharides - pharmacology, Pretreatment, RNA, Plant - genetics, Saccharification, Stems, Sugar, Sugars, Tissue Engineering - methods, Transgenic plants, Wheat