Details

Autor(en) / Beteiligte

• Albert, Thomas K
• Grote, Korbinian
• Boeing, Stefan
• Stelzer, Gertraud

Titel

Global distribution of negative cofactor 2 subunit-[alpha] on human promoters

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2007-06, Vol.104 (24), p.10000

Ort / Verlag

Washington: National Academy of Sciences

Erscheinungsjahr

2007

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Negative cofactor 2 (NC2) forms a stable complex with TATA- binding protein (TBP) on promoters in vitro. Its association with TBP prevents the binding of TFIIB and leads to inhibition of preinitiation complex formation. Here, we investigate the association of NC2 subunit-α with human RNA polymerase TI promoter regions by using gene-specific ChIP and genome-wide promoter ChlPchip analyses. We find NC2a associated with a large number of human promoters, where it peaks close to the core regions. NC2 occupancy in vivo positively correlates with mRNA levels, which perhaps reflects its capacity to stabilize TBP on promoter regions. In single gene analyses, we confirm core promoter binding and in addition map the NC2 complex to enhancer proximal regions. High-occupancy histone genes display a stable NC2/TFTTB ratio during the cell cycle, which otherwise varies markedly from one gene to another. The latter is at least in part explained by an observed negative correlation of NC2 occupancy with the presence of the TFTTB recognition element in core promoter regions. Our data establish the genome-wide basis for general and gene-specific functions of NC2 in mammalian cells. [PUBLICATION ABSTRACT]