Proceedings of the National Academy of Sciences - PNAS, 2004-03, Vol.101 (11), p.3880-3885
2004
Details
- Autor(en) / Beteiligte
- Titel
- Interleukin 2 Plays a Central Role in Th2 Differentiation
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2004-03, Vol.101 (11), p.3880-3885
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2004
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Differentiation of naïve CD4 T cells into T helper (Th) 2 cells requires signaling through the T cell receptor and an appropriate cytokine environment. IL-4 is critical for such Th2 differentiation. We show that IL-2 plays a central role in this process. The effect of IL-2 on Th2 generation does not depend on its cell growth or survival effects. Stat5 a-/-cells show diminished differentiation to IL-4 production, and forced expression of a constitutively active form of Stat5a replaces the need for IL-2. In vivo IL-2 neutralization inhibits IL-4 production in two models. Studies of restriction enzyme accessibility and binding of Stat5 to chromatin indicate that IL-2 mediates its effect by stabilizing the accessibility of the Il4 gene. Thus, IL-2 plays a critical role in the polarization of naive CD4 T cells to the Th2 phenotype.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.0400339101Titel-ID: cdi_proquest_journals_201358275
- Format
- –
- Schlagworte
- Animals, Antigen presenting cells, Binding sites, Biological Sciences, CD4 antigen, Cell culture techniques, Cell cycle, Cell Differentiation - physiology, Cell growth, Cultured cells, Cytokines, Dendritic cells, DNA-Binding Proteins - metabolism, GATA3 Transcription Factor, Immunology, Infections, Interleukin-2 - metabolism, Interleukin-4 - genetics, Interleukin-4 - metabolism, Life Sciences, Lymph nodes, Mice, Milk Proteins, Retroviridae, STAT5 Transcription Factor, Stat5a protein, T lymphocytes, Th2 Cells - metabolism, Trans-Activators - metabolism