Details

Autor(en) / Beteiligte

• Hanchard, Julia Ann

Titel

Functional Enzyme Discovery by LC-MS Metabolomics in Yeast

Ort / Verlag

ProQuest Dissertations & Theses

Erscheinungsjahr

2015

Quelle

ProQuest Dissertations & Theses A&I

Beschreibungen/Notizen

• In pursuit of novel enzymes, I have carried out a systematic analysis of gene function in the budding yeast, Saccharomyces cerevisiae. I used LC-MS metabolomics to profile metabolite levels for 120 prototrophic mutants carrying deletions in uncharacterized candidate enzyme-encoding genes. Methodology I developed in this study was successful in capturing metabolomic phenotypes for 26 candidate enzyme deletion mutants. Furthermore, I explored the function of a set of mutants deleted for genes not previously known to have a role in metabolism. I showed these mutants have altered levels of NAD+ biosynthetic intermediates. New phenotypes, here discovered, will lead to dissection of biochemical functions of uncharacterized genes and determination of their contributions to metabolism. I have also uncovered biologically-derived compounds that are uncharacterized and may be new metabolites. Overall, my work underscores LC-MS metabolomics as a powerful tool for functional genomics in its ability capture the metabolic functions of uncharacterized genes.