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Development of cultures of the marine sponge Hymeniacidon perleve for genotoxicity assessment using the alkaline comet assay
Environmental toxicology and chemistry, 2017-12, Vol.36 (12), p.3314-3323
Akpiri, Rachael U.
Konya, Roseline S.
Hodges, Nikolas J.
2017
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Akpiri, Rachael U.
Konya, Roseline S.
Hodges, Nikolas J.
Titel
Development of cultures of the marine sponge Hymeniacidon perleve for genotoxicity assessment using the alkaline comet assay
Ist Teil von
Environmental toxicology and chemistry, 2017-12, Vol.36 (12), p.3314-3323
Ort / Verlag
United States: Blackwell Publishing Ltd
Erscheinungsjahr
2017
Quelle
Wiley-Blackwell Full Collection
Beschreibungen/Notizen
Sponges are a potential alternative model species to bivalves in pollution biomonitoring and environmental risk assessment in the aquatic ecosystem. In the present study, a novel in vivo exposure sponge culture model was developed from field‐collected and cryopreserved sponge (Hymeniacidon perleve) cells to investigate the genotoxic effects of environmentally relevant metals in the laboratory. Sponge cell aggregates were cultured and exposed to noncytotoxic concentrations (0–0.4 mg/L) of cadmium chloride, nickel chloride, and sodium dichromate as quantified by the reduction of 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and DNA‐strand breaks assessed by the comet assay. Reactive oxygen species (ROS) formation was quantified by oxidation of 2′,7′‐dichlorofluorescin diacetate in sponge cell aggregates exposed to the same concentrations of Cd, Cr, and Ni. There was a statistically significant (p < 0.05) concentration‐dependent increase in the level of DNA‐strand breaks and ROS formation in all of the metals investigated. To the best of our knowledge, we have utilized for the first time the alkaline comet assay to detect DNA‐strand breaks in marine sponge cells and demonstrated that exposure to noncytotoxic concentrations of Cd, Cr, and Ni for 12 h results in a concentration‐dependent increase in DNA damage and levels of ROS production. In conclusion, we have developed a novel in vivo model based on culture of cryopreserved sponge cells that is compatible with the alkaline comet assay. Genotoxicity in marine sponges measured by the comet assay technique may be a useful tool for biomonitoring research and risk assessment in aquatic ecosystems. Environ Toxicol Chem 2017;36:3314–3323. © 2017 SETAC
Sprache
Englisch
Identifikatoren
ISSN: 0730-7268
eISSN: 1552-8618
DOI: 10.1002/etc.3907
Titel-ID: cdi_proquest_journals_1968583663
Format
–
Schlagworte
Aggregates
,
Animals
,
Aquatic ecosystems
,
Bioassays
,
Biomonitoring
,
Cadmium
,
Cadmium chloride
,
Cadmium Chloride - toxicity
,
Cell culture
,
Chromates
,
Chromates - toxicity
,
Chromium
,
Comet assay
,
Comet Assay - methods
,
Cryopreservation
,
Culture Techniques - methods
,
Damage detection
,
Deoxyribonucleic acid
,
DNA
,
DNA Breaks
,
DNA damage
,
Ecological risk assessment
,
Ecosystem assessment
,
Genotoxicity
,
Heavy metal
,
Heavy metals
,
Hymeniacidon perleve
,
In vivo methods and tests
,
Marine sponge
,
Mollusks
,
Nickel
,
Nickel - toxicity
,
Nickel chloride
,
Oxidation
,
Porifera - drug effects
,
Porifera - genetics
,
Reactive oxygen species
,
Reactive Oxygen Species - metabolism
,
Risk assessment
,
Shellfish
,
Sodium
,
Sodium dichromate
,
Sponge culture
,
Sponges
,
Statistical analysis
,
Studies
,
Water Pollutants, Chemical - toxicity
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