Details

Autor(en) / Beteiligte

• Xia, Jie
• Zhuang, Yu-Ting
• Yu, Yong-Liang
• Wang, Jian-Hua

Titel

Highly fluorescent carbon polymer dots prepared at room temperature, and their application as a fluorescent probe for determination and intracellular imaging of ferric ion

Ist Teil von

• Mikrochimica acta (1966), 2017-04, Vol.184 (4), p.1109-1116

Ort / Verlag

Vienna: Springer Vienna

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Carbon polymer dots (CPDs) were prepared by a one-pot aqueous synthetic route from ascorbic acid and diethylenetriamine at room-temperature. The CPDs under 350-nm excitation exhibit blue fluorescence peaking at 430 nm with a quantum yield of 47%. Other features include an average diameter of 5 nm, a fluorescence that is independent of the excitation wavelength, good water dispersibility and photostability, and excellent biocompatibility. The CPDs are shown to be viable fluorescent probes for ferric ion which acts as a strong quencher. The response to Fe(III) is linear in the 0.2 to 10 μM concentration range, and the detection limit is 0.1 μM. The probe was applied to the determination of Fe(III) in environmental waters and to intracellular imaging of ferric ions in HeLa cells. Graphical abstract Carbon polymer dots (CPDs) are prepared from ascorbic acid and diethylenetriamine (DETA) at room-temperature (RT). The RT-CPDs exhibit excellent optical performance, biocompatibility and selectivity of quenching by ferric ions. This can be applied for determination and intracellular imaging of ferric ion.