Details

Autor(en) / Beteiligte

• Morawiecka, B
• Lorenc-Kubis, I
• Wieczorek, E
• Kubicz, A

Titel

Comparative studies on the carbohydrate, protein and acid phosphatase contents in seeds of some rye (Secale cereale) varieties

Ist Teil von

• Acta Societatis Botanicorum Poloniae, 2015-01, Vol.45 (1/2), p.111-117

Ort / Verlag

Warsaw: Polish Botanical Society

Erscheinungsjahr

2015

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The contents of sugars. proteins and acid phosphatase extracted with 0.1 M acetate buffer, pH 5.1, from some rye varieties were determined. The total sugar level amounted to 3.25-9.70 g per 100 g of seeds; the estimates for pentoses were 1.7-2.9 g and those for proteins 0.91-1.60 g per 100 g of seeds. Acid phosphatase showed and activity level between 0.18 and 1.26 units/mg protein. After disc electrophoresis proteins were separated into 10 to 11 bands: at pH 9.4 or into 4-7 bands at pH 3.8. Essential variety differences were expressed in protein patterns after electrophoresis at pH 3.8. Acid phosphatase was separated into 5 and 4 activity bands at pH 9.4 and 3.3,. respectively. No variations in zymogram patterns were observed in respect to variety differences or cultivation in various climate and soil conditions.