Details

Autor(en) / Beteiligte

• Raouafi, Amal
• Rabti, Amal
• Raouafi, Noureddine

Titel

A printed SWCNT electrode modified with polycatechol and lysozyme for capacitive detection of α-lactalbumin

Ist Teil von

• Mikrochimica acta (1966), 2017-11, Vol.184 (11), p.4351-4357

Ort / Verlag

Vienna: Springer Vienna

Erscheinungsjahr

2017

Link zum Volltext

Quelle

springer (창간호~2014)

Beschreibungen/Notizen

• The authors describe an electrochemical sensor for the breast cancer marker α-lactalbumin (αLA). It is based on the use of printed single-walled carbon nanotube (SWCNT) electrodes that were modified with polycatechol. Impedance-derived electrochemical capacitance spectroscopy (ECS) is applied for detection at an applied potential of −0.14 V vs. Ag/AgCl reference electrode. The electrode was prepared in a two-step process. First, a dispersion of SWCNTs was drop-cast onto the surface of a poly(ethylene terephthalate) substrate to act as the working electrode. Next, catechol was electrochemically polymerized on the SWCNTs, prior to the immobilization of lysozyme. The strong interaction between lysozyme and αLA induced changes in the redox capacitance which are detected by ECS. The latter shows the device to be capable of detecting αLA in the 20 to 80 ng·mL −1 concentration range. The limit of detection is 9.7 ng·mL −1 at an S/N ratio of 3. The device was used to detect αLA in human blood serum with good recovery results. Graphical abstract A sensitive biosensor for αLA was prepared by modifying SWCNT electrode with polycatechol and lysozyme. The electrochemical capacitance spectroscopy was used for the first time to selectively detect αLA in the blood in the range from 20 to 80 ng·mL −1 .