Details

Autor(en) / Beteiligte

• Chen, Rong
• Lv, Ruichen
• Xiao, Lisheng
• Wang, Min
• Du, Zongmin
• Tan, Yafang
• Cui, Yujun
• Yan, Yanfeng
• Luo, Yanping
• Yang, Ruifu
• Song, Yajun

Titel

A1S_2811, a CheA/Y‐like hybrid two‐component regulator from Acinetobacter baumannii ATCC 17978, is involved in surface motility and biofilm formation in this bacterium

Ist Teil von

• MicrobiologyOpen (Weinheim), 2017-10, Vol.6 (5)

Ort / Verlag

Bognor Regis: John Wiley & Sons, Inc

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Wiley HSS Collection

Beschreibungen/Notizen

• Abstract Two‐component systems in Acinetobacter baumannii are associated with its virulence, drug resistance, motility, biofilm formation, and other characteristics. In this study, we used Rec Ab , a genetic engineering method, to investigate the function of A1S_2811 in A. baumannii strain ATCC 17978. A1S_2811, a hypothetical hybrid sensor histidine kinase/response regulator, has four histidine‐containing phosphotransfer domains, a CheA‐like regulatory domain, and a CheY‐like receiver domain at its C terminus. Compared with the ATCC 17978 strain, both surface motility and biofilm formation at the gas–liquid interface decreased significantly in the A1S_2811 knock‐out strain. The number of pilus‐like structures and the amount of extrapolymeric substances on the cell surface also decreased in the A1S_2811 null strain. Transcription of abaI , which encodes an N ‐acylhomoserine lactone synthase in A. baumannii , decreased significantly in the A1S_2811 null strain, and supplementation with synthetic N ‐(3‐oxodecanoyl) homoserine‐ l ‐lactone rescued the surface motility and biofilm formation phenotype in the null mutant. We speculate that A1S_2811 regulates surface motility and biofilm formation, not by regulating type IV pili‐associated genes expression, but by regulating the chaperone/usher pili‐associated csuA/ ABCDE operon and the AbaI‐dependent quorum‐sensing pathway‐associated A1S_0112‐0119 operon instead.