Details

Autor(en) / Beteiligte

• Zhu, Bao-Hua
• Tu, Chang-Chao
• Shi, Hong-Ping
• Yang, Guan-Pin
• Pan, Ke-Hou

Titel

Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum

Ist Teil von

• Process biochemistry (1991), 2017-06, Vol.57, p.43-49

Ort / Verlag

Barking: Elsevier Ltd

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Overexpression of endogenous D6 desaturase gene increased markedly their EPA content.•Overexpression of endogenous D6 desaturase gene resulted in increase in total lipids.•D6 desaturase is a key enzyme for EPA biosynthesis.•Overexpression of endogenous Δ6D is a valid way to improve EPA production in microalgae. The effect of overexpression of endogenous delta-6 fatty acid desaturase gene (ER 6FAD) on eicosapentaenoic acid (EPA) production and total lipid content was investigated in Phaeodactylum tricornutum. All three randomly selected transformants exhibited significant increase (47.66%) in their EPA content, which reached up to 38.101mg/g DW in algal strain D63. The total lipid content of all the three transformants was significantly higher (16.40–18.64%) than that of the wild-type strain. These findings suggested that the reaction catalyzed by ER 6FAD is a limiting step for EPA biosynthesis, overexpression of endogenous ER 6FAD gene is an effective method for improving EPA production in eukaryotic microalgae, and fatty acid metabolic pathway in microalgae can be genetically modified.