Details

Autor(en) / Beteiligte

• Artykov, A. A.
• Fursova, K. K.
• Ryazantsev, D. Yu
• Shchannikova, M. P.
• Loskutova, I. V.
• Shepelyakovskaya, A. O.
• Laman, A. G.
• Zavriev, S. K.
• Brovko, F. A.

Titel

Detection of staphylococcal enterotoxin a by phage display mediated immuno-PCR method

Ist Teil von

• Russian journal of bioorganic chemistry, 2017-09, Vol.43 (5), p.540-543

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phage display mediated immuno-PCR method is 100 pg/mL that is much lower than the level of toxic and allergic dose.