Details

Autor(en) / Beteiligte

• Kulkarni, H S
• Elvington, M L
• Liszewski, M
• Brody, S L
• Atkinson, J P

Titel

C31 BASIC AND TRANSLATIONAL STUDIES ON THE ROLE OF THE EPITHELIUM: Human Airway Epithelial Cells Have Multiple Sources And Stores Of The Intracellular Complement Protein C3

Ist Teil von

• American journal of respiratory and critical care medicine, 2017-01, Vol.195

Ort / Verlag

New York: American Thoracic Society

Erscheinungsjahr

2017

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• BEAS-2B and hTEC cultured in serum-free media (C3-free) had a 190-kDa protein corresponding to purified C3 that was not present in cell lines such as Farage (B-cell), Jurkat (T-cell) and ARPE (retina). On treatment of BEAS2B and hTEC cell lysates with dithiothreitol (DTT), only a fraction of the 190-kDa protein reduced to the C3 alpha- and beta-chain as determined by protein standards, while the rest remained at 190-kDa, consistent with the precursor form (pro-C3). Research reported in this publication is supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases, part of the National Institutes of Health, under Award Number P30AR048335 and the National Institutes of Health Grant for the Washington University Institute of Clinical and Translational Sciences (3UL1TR000448).