Details

Autor(en) / Beteiligte

• Funke, S.
• Perumal, N.
• Schmelter, C.
• Teister, J.
• Markowitsch, S.
• Beck, S.
• Pfeiffer, N.
• Grus, F.H.

Titel

Retina proteomics provide new insights in glaucoma

Ist Teil von

• Acta ophthalmologica (Oxford, England), 2016-10, Vol.94 (S256), p.n/a

Ort / Verlag

Malden: Wiley Subscription Services, Inc

Erscheinungsjahr

2016

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Summary Proteomic alterations have been studied in retina samples of glaucoma and non‐glaucoma control donor eyes (N = 5/group) by use of a bottom up proteomic platform implementing LC ESI LTQ Orbitrap XL MS analysis and label‐free quantification following functional analysis using gene ontology (GO) annotation. Furthermore, candidate abundances were examined in porcine retina and optic nerve head preparations (N = 12/tissue type). Approximately 10% of identified proteins (>600, FDR < 1%) showed significant level alteration (p < 0.05) or distinct tendencies in glaucomatous retinae, predominantly encircling mitochondrial and nucleus residing proteins. Thereby, new candidates, e.g. ADP/ATP translocase 3 and methyl‐CpG‐binding protein 2 could be proposed to be associated to glaucoma. Numerous candidates, e.g. retinol‐binding protein 3 show characteristic distribution of abundance comparing optic nerve head and retina proteomes in porcine model eyes (p < 0.05). In conclusion, distinct proteomic alterations could be documented in the human glaucomatous retina highlighting new retinal protein candidates. Moreover, characteristic distribution of ocular proteins and candidates could be revealed.