Biocontrol Science, 2017, Vol.22(2), pp.97-104
2017
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Details
- Autor(en) / Beteiligte
- Titel
- Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method
- Ist Teil von
- Biocontrol Science, 2017, Vol.22(2), pp.97-104
- Ort / Verlag
- Japan: The Society for Antibacterial and Antifungal Agents, Japan
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction. In this study, we evaluated the correlation between colony forming units (CFU) and LAMP detection rate using the LAMP method for environmental fungi. The LAMP method should be a useful means of detecting fungi in indoor environments, disaster areas, or even in confined manned spacecraft to prevent allergies or infections caused by fungi.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1342-4815eISSN: 1884-0205DOI: 10.4265/bio.22.97Titel-ID: cdi_proquest_journals_1916094271
- Format
- –
- Schlagworte
- Alignment, Allergic reactions, Allergies, Basidiomycota - genetics, Basidiomycota - isolation & purification, Candida - genetics, Candida - isolation & purification, Colony-forming cells, Correlation, Correlation analysis, Cryptococcus - genetics, Cryptococcus - isolation & purification, Deoxyribonucleic acid, Detection, Detection of fungi, DNA, DNA Primers - genetics, Environmental fungi, Food Microbiology - methods, Fungal infections, Fungal Proteins - genetics, Fungi, Gene sequencing, Indoor environments, Loop-mediated isothermal amplification (LAMP), Manned spacecraft, Methods, Nucleic Acid Amplification Techniques - methods, Nucleic acids, Procedure for Ultra Rapid Extraction (PURE), Ribonucleic acid, RNA, rRNA, Sensitivity and Specificity, Spacecraft, Specificity, Temperature, Temperature effects, Thermal cycling