Details

Autor(en) / Beteiligte

• Salam, S
• Lekshmi, S
• Silvester, R
• Krishnan, K P
• Saramma, A V
• Hatha, A A M

Titel

Effect of environmental factors on growth and enzyme production of cold adapted bacteria from water and sediment of Kongsfjord, Ny-Alesund, Arctic

Ist Teil von

• Journal of environmental biology, 2017-07, Vol.38 (4), p.579

Ort / Verlag

Lucknow: Triveni Enterprises

Erscheinungsjahr

2017

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• The Arctic region has several distinct habitats which makes Arctic a potential region for identifying novel microbial extremophiles. The main objective of the study was to find out the effect of environmental factors such as temperature, pH and salinity on the growth and hydrolytic enzyme production capabilities of psychrotrophic bacteria from the water and sediment of Kongsfjord, Arctic. One hundred and twenty two bacterial isolates from water and sediment of Kongsfjord (79°58'N, 12°E) was obtained for the study. Bacterial isolates were inoculated on ZoBell's Marine medium to study the effect of temperature (4 °C, 20 °C and 37 °C), pH (2-13) and salinity (0.5-4 M) on growth. Effect of temperature on production of extracellular enzymes such as amylase, gelatinase and lipase were checked by plate assay. All the isolates showed growth at 4 °C, 20 °C and 37 °C; signifying that these isolates were psychrotolerants rather than true psychrophiles. More than 50% of isolates from both water and sediment samples showed growth at wide pH (2-11) range. Salinity tolerance for majority of isolates (54%) of water was 3M and that from sediment varied from 0.5 M to 4 M. Majority of the isolates were capable of producing lipase, followed by gelatinase and amylase. Nearly, all the isolates from water and sediment showed lipolytic activity at 37 °C and more than 70% of isolates at 4°C. All the isolates produced one or more enzymes; amylase, lipase or protease at 4 °C and (or) 37 °C and showed growth at wide pH range and exhibited high salinity tolerance. Thus, the bacteria present in water and sediments of Kongsford are a potential source of novel hydrolytic enzymes, especially lipase that have wide applications in biotechnology, medicine, agriculture, etc.