Details

Autor(en) / Beteiligte

• He, Shiyi
• Wang, Aiyan
• Ning, Xueping
• Yang, Dongjun
• Ling, Min

Titel

The protective effect of a novel antioxidant gene from Mycobacterium avium against nitrosative and oxidative stress in E. coli

Ist Teil von

• World journal of microbiology & biotechnology, 2017-06, Vol.33 (6), p.127, Article 127

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• The production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) is an important host defense mechanism in response to infection by Mycobacterium tuberculosis . A variety of genes have been implicated in resistance to ROI and RNI, including noxR1 . However, studies in Mycobacterium avium , an important pathogen among nontuberculous mycobacteria , are limited. We aim to investigate the role of a novel gene cloned from M. avium with high similarity to noxR1, noA , in resistance against RNI and ROI in M. tuberculosis . After subcloning noA into vector for expression in E. coli , we performed survival rate analysis in the bacteria transformed with noA (pET-noA) and without noA (pET-his) after exposure to nitrosative stresses by S-nitrosoglutathione (GSNO) and sodium nitrite, and oxidative stresses by H 2 O 2 . Compared with pET-his, the survival rate of pET-noA was 1 log 10 -fold higher after exposure to GSNO and sodium nitrite. We observed 1 log 10 -fold, 2 log 10 -fold and 3 log 10 -fold higher survival rate in pET-noA than pET-his after exposure to H 2 O 2 for 3, 6 and 9 h, respectively. With the combined treatment of H 2 O 2 and GSNO, we found more than 2 log 10 -fold increase in survival rate in pET-noA comparing with pET-his, suggesting a possible synergistic effect. In summary, noA gene cloned from M. avium has been shown to protect E. coli from both RNI and ROI.