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The protective effect of a novel antioxidant gene from Mycobacterium avium against nitrosative and oxidative stress in E. coli
Ist Teil von
World journal of microbiology & biotechnology, 2017-06, Vol.33 (6), p.127, Article 127
Ort / Verlag
Dordrecht: Springer Netherlands
Erscheinungsjahr
2017
Quelle
MEDLINE
Beschreibungen/Notizen
The production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) is an important host defense mechanism in response to infection by
Mycobacterium tuberculosis
. A variety of genes have been implicated in resistance to ROI and RNI, including
noxR1
. However, studies in
Mycobacterium avium
, an important pathogen among
nontuberculous mycobacteria
, are limited. We aim to investigate the role of a novel gene cloned from
M. avium
with high similarity to
noxR1, noA
, in resistance against RNI and ROI in
M. tuberculosis
. After subcloning
noA
into vector for expression in
E. coli
, we performed survival rate analysis in the bacteria transformed with
noA
(pET-noA) and without
noA
(pET-his) after exposure to nitrosative stresses by S-nitrosoglutathione (GSNO) and sodium nitrite, and oxidative stresses by H
2
O
2
. Compared with pET-his, the survival rate of pET-noA was 1 log
10
-fold higher after exposure to GSNO and sodium nitrite. We observed 1 log
10
-fold, 2 log
10
-fold and 3 log
10
-fold higher survival rate in pET-noA than pET-his after exposure to H
2
O
2
for 3, 6 and 9 h, respectively. With the combined treatment of H
2
O
2
and GSNO, we found more than 2 log
10
-fold increase in survival rate in pET-noA comparing with pET-his, suggesting a possible synergistic effect. In summary,
noA
gene cloned from
M. avium
has been shown to protect
E. coli
from both RNI and ROI.