Details

Autor(en) / Beteiligte

• Tsubokura, Kazuki
• Vong, Kenward K. H.
• Pradipta, Ambara R.
• Ogura, Akihiro
• Urano, Sayaka
• Tahara, Tsuyoshi
• Nozaki, Satoshi
• Onoe, Hirotaka
• Nakao, Yoichi
• Sibgatullina, Regina
• Kurbangalieva, Almira
• Watanabe, Yasuyoshi
• Tanaka, Katsunori

Titel

In Vivo Gold Complex Catalysis within Live Mice

Ist Teil von

• Angewandte Chemie International Edition, 2017-03, Vol.56 (13), p.3579-3584

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–AuIII complex was designed and developed that enables organ‐specific, localized propargyl ester amidation with nearby proteins within live mice. The targeted reactivity can be imaged through the use of Cy7.5‐ and TAMRA‐linked propargyl ester based fluorescent probes. This targeting system could enable the exploitation of other metal catalysis strategies for biomedical and clinical applications. The first metal‐catalyzed reaction that proceeds within live mice is based on a targeting approach with glycans. Glycoalbumin–AuIII complexes can be accumulated in specific organs where they catalyze amide bond formation between a propargyl ester probe and amine groups on nearby proteins. The selective targeting was confirmed by whole body fluorescence imaging and analysis of dissected tissues.