Details

Autor(en) / Beteiligte

• Agarwal, Nayan P.
• Matthies, Michael
• Gür, Fatih N.
• Osada, Kensuke
• Schmidt, Thorsten L.

Titel

Block Copolymer Micellization as a Protection Strategy for DNA Origami

Ist Teil von

• Angewandte Chemie International Edition, 2017-05, Vol.56 (20), p.5460-5464

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• DNA nanotechnology enables the synthesis of nanometer‐sized objects that can be site‐specifically functionalized with a large variety of materials. For these reasons, DNA‐based devices such as DNA origami are being considered for applications in molecular biology and nanomedicine. However, many DNA structures need a higher ionic strength than that of common cell culture buffers or bodily fluids to maintain their integrity and can be degraded quickly by nucleases. To overcome these deficiencies, we coated several different DNA origami structures with a cationic poly(ethylene glycol)–polylysine block copolymer, which electrostatically covered the DNA nanostructures to form DNA origami polyplex micelles (DOPMs). This straightforward, cost‐effective, and robust route to protect DNA‐based structures could therefore enable applications in biology and nanomedicine where unprotected DNA origami would be degraded. Polymeric protection: DNA origami nanostructures generally need higher ionic strengths than those of common cell culture buffers to maintain their integrity and are quickly degraded by nucleases. They can, however, be protected from these processes through the formation of polyplex micelles with poly(ethylene glycol)‐b‐polylysine.