Details
- Autor(en) / Beteiligte
- Titel
- Purification and preparation of ginkgo acid monomers and the study of its analytical method
- Ort / Verlag
- ProQuest Dissertations & Theses
- Erscheinungsjahr
- 2011
- Quelle
- ProQuest Dissertations & Theses A&I
- Beschreibungen/Notizen
- Translation from original language as provided by authorBecause ginkgolic acid(GKA) has allergenicity, embryo toxicity, immune toxicity and cell toxicity, so it has been considered as the main side effects constituents in EGb and its preparation. Its concentration has been restricted to 5 ppm and has become a major control target of the quality of Ginkgo. The aim of this subject is to establish purification and preparation methods of high purity GKA monomers and analysis methods of GKA. In this paper, crude GKA extract which was extracted from sarcotestas of Ginkgo biloba was purified by middle pressure silica gel column and the total GKA was obtained. Further the total GKA was separated by preparative high performance liquid chromatography to obtain GKA monomers and their purity and structure had been analysed. Two analysis methods of GKA by UPLC and LC/MS/MS had been discussed. The effect of factors such as solvent, eluent, flow rate and sample amount had been studied to obtain the optimal conditions: using
- Sprache
- Chinesisch
- Identifikatoren
- Titel-ID: cdi_proquest_journals_1869197404
- Format
- –
- Schlagworte
- Chemical engineering