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The research in anti-tumor activity of the novel gsk-3β inhibitor-polypyridyl ruthenium complexes
Ort / Verlag
ProQuest Dissertations & Theses
Erscheinungsjahr
2011
Link zum Volltext
Quelle
ProQuest Dissertations & Theses A&I
Beschreibungen/Notizen
Translation from original language as provided by authorObjective: Aim to screen the optimal polypyridyl ruthenium complexes with the best activity on human glioma SWO-38 cells. Further, to investigate the possible mechanism of the anti-tumor activity of chiral ruthenium complexes inhibiting GSK-3β activity in vitro. Methods: 1. To screen the optimal complexes with highest activity and lowest toxicity from eight kinds of polypyridyl ruthenium complexes with chiral separation by MTT colorimetric assay. And detecting the proliferation of the cells with different concentrations of the complexes. The location of complexes in the cells could be observed under the confocal laser scanning microscope. 2. FITC-Annexin V/PI double labelling was used to detect the apoptosis of SWO-38 cells; 3. Western blot was employed to detect the expression of total GSK-3β, p-GSK-3βSer9, total PTEN and p-PTEN Ser380/Thr382/383 after the treatment of the optimal complexes, then analyzing the mechanism of the inhibition proliferation o