Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Molecular detection and typing and analysis of complete sequence of pathogens associated with duck viral hepatitis
Ort / Verlag
ProQuest Dissertations & Theses
Erscheinungsjahr
2009
Quelle
ProQuest Dissertations & Theses A&I
Beschreibungen/Notizen
Translation from original language as provided by authorDuck viral hepatitis can be caused by duck hepatitis A virus (DHAV) and duck astrovirus (DAstV). This study was performed to develop a new approach for detecting and typing of DHAV directly from clinical samples and to investigate the genotypes circulating in China. In addition, the complete sequence of a DAstV strain presented in a DHAV-negative sample was determined. The genomic organization of DAstV and the relationship between DAstV and other astroviruses was further analyzed. Based on the comparison of genomic sequences of known DHAVs, a pair of primers was designed targeting the full conserved regions in 5 UTR and the DHAV-specific RT-PCR was therefore developed. An approximately 250-bp 5 UTR sequence was successfully amplified from DHAV strains, involving 2 serotypes. By determining the nucleotide sequence of the amplion, a molecular typing method was developed. If isolate sequences were compared to 5 UTR sequences available in public databases, n