Details

Autor(en) / Beteiligte

• Makwana, Nandini B
• Foley, Bree
• Lee, Silvia
• Fernandez, Sonia
• Irish, Ashley B
• Price, Patricia

Titel

Asymptomatic CMV infections in long-term renal transplant recipients are associated with the loss of FcR[gamma] from LIR-1+ NK cells

Ist Teil von

• European journal of immunology, 2016-11, Vol.46 (11), p.2597

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2016

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• While it is established that cytomegalovirus (CMV) disease affects NK-cell profiles, the functional consequences of asymptomatic CMV replication are unclear. Here, we characterize NK cells in clinically stable renal transplant recipients (RTRs; n = 48) >2 years after transplantation. RTRs and age-matched controls (n = 32) were stratified by their CMV serostatus and the presence of measurable CMV DNA. CMV antibody or CMV DNA influenced expression of NKG2C, LIR-1, NKp30, NKp46, and FcR[gamma], a signaling adaptor molecule, on CD56dim NK cells. Phenotypic changes ascribed to CMV were clearer in RTRs than in control subjects and affected NK-cell function as assessed by TNF-[alpha] and CD107a expression. The most active NK cells were FcR[gamma]-LIR-1+NKG2C- and displayed high antibody-dependent cell cytotoxicity responses in the presence of immobilized CMV glycoprotein B reactive antibody. However, perforin levels in supernatants from RTRs with active CMV replication were low. Overall we demonstrate that CMV can be reactivated in symptom-free renal transplant recipients, affecting the phenotypic, and functional profiles of NK cells. Continuous exposure to CMV may maintain and expand NK cells that lack FcR[gamma] but express LIR-1.